C1單細胞 基因製備系統
型號:
應用範圍:
Single-Cell Gene Expression, mRNA sequencing, miRNA analysis, Somatic DNA Sequencing

規格/特色說明




微量單細胞定序基因製備儀
C1

藉由拋棄式微流道晶片針對單一細胞進行獨立分離,單一細胞於晶片中可完成lysisRNA purificationReverse transcriptionpre-amplify,最後將單一細胞之終產物流放之單一細胞槽中,可回收做進一步分析


 
晶片選擇

晶片之應用包含DNA Seq, mRNA SeqmRNA Seq HT。針對不同細胞大小可選擇5–10µm,10–17 μm, 17-25µm三種不同的晶片。

 

C1 chip for DNA sequence

C1 chip for mRNA sequence







    

C1™ Single-Cell Auto Prep System Technical Overview

C1™ Single-Cell AutoPrep Array™ IFC Workflow






Gene Expression




mRNA Sequening




miRNA Analysis


參考文獻

Customer Publications Featuring Fluidigm Single-Cell Technology: BibliographyTrapnell, C. et al. “The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells.” Nature Biotechnology 32(4) (2014): 381–6.*

Armond, J. et al. “A stochastic model dissects cell states in biological transition processes.” Scientific Reports 4 (2014): 3,692.

Navin, N. et al. “The first five years of single-cell cancer genomics and beyond.” Genome Research 25 (10) (2015):1499–507.*

Ozkumur, E. et al. “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells.” Science Translational Medicine 5(179) (2013): 179ra47.

Powell, A.A. et al. “Single cell profiling of circulating tumor cells: transcriptional heterogeneity and diversity from breast cancer cell lines.” PLoS One 7 (2012): (5):e33788.

Helzer, K.T. et al. “Circulating tumor cells are transcriptionally similar to the primary tumor in a murine prostate model.” Cancer Research 69(19) (2009): 7,860–6.




原廠網址:https://www.fluidigm.com/products/c1-system
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